Mykola Blazheyevskiy, Olena Kovalska


Authors have offered a novel colorimetric biosensor for determination of acetylcholinesterase (AChE) activity and its inhibitors on the example of cationic surfactant Benzalkonium chloride (BAC) in aqueous solutions by taking utilization of H2O2 – 4- ethoxyaniline (p-Ph) detection system. In the presence of AChE, acetylcholine (ACh) was hydrolyzed to choline and acetic acid. H2O2 could interact with unreacted Ach, and in situ formed CH3CO3H can oxidize p-Ph to azoxyphenetole (ox p-Ph), resulting in the developing light brown colour and improvement of absorbance at 350 nm. A colorimetric method, developed for estimating the acetylcholinesterase activity using ACh as the substrate, measures the rate of ox- p-Ph formation and assay of the cationic surfactant BAC, being highly sensitive with a lower detection limit at 6∙10-7 mol/L. The obtained assay is fairly simple, inexpensive, which may be used for screening the trace amount of cationic surfactants.

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DOI: http://dx.doi.org/10.18007/gisap:pmc.v0i8.1550


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