Andrey Korshunov, Igor Shestel, Michail Hamov, Sergey Markovskiy, Sergey Moskovskiy, Vladimir Konev


The purpose of the work: to study opportunities of application of atomic-force microscopy for morphological diagnostics of the pathology of the connecting tissue on firm tissues of teeth and bones.
Materials and research methods: the analysis of the packing extent, size and form of bone plates, enamel prisms, size of an inter-prismatic interval and its height on 30 removed teeth and the bone tissue of persons with a connecting tissue dysplasia and 27 removed teeth and the bone tissue of a control group by the nuclear-power microscopy method.
Results: research of a bone tissue and enamel of teeth is carried out in conditions of the connecting tissue pathology in comparison with patients without it. It was established that collagen configuration in conditions of the connecting tissue pathology assumes increase of an interval between fibers up to 98 nm which reduce the quantitative content of the mineral matrix in a bone. In enamel of teeth we have also determined violations of forms and sizes of enamel prisms (5.5х5.4 microns) with increasing distance between enamel prisms up to 1.6 micron.
Conclusions: 1. Results of examination of the ultra-structure and mineral structure allow to talk about violation of mineralization and organization of enamel of teeth and the bone tissue of persons with the connecting tissue pathology symptoms. 2. Use of the nuclear-power microscopy allows to study native cellular cultures, including firm tissue of teeth and the bone tissue. 3. The specified facts can be used as a basis for the diagnostics of the connecting tissue pathology and for determination of individual characteristics in the process of identification of the personality.

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