APPLICATION OF THE KINETIC ENZYMATIC METHOD FOR BENZALKONIUM CHLORIDE DETERMINATION IN AN AEROSOL PREPARATION

Mykola Blazheyevskiy, Olena Koval’ska

Abstract


A new kinetic photometric method for BAC determination based on inhibition of the enzymatic (cholinesterase) reaction was proposed. The reaction rate was detected at unhydrolised acetylcholine residue, which is determined by the amount of peracetic acid, produced during the impact of H2O2 on it. Indicator reaction is a reaction of peracetic acid with 4-ethoxyaniline interaction that leads to the formation of azoxyphenetole with λmax = 358 nm (lgε =4.18). The measurement of the velocity of changes in the light absorption vs. time (∆А/∆t, min-1) gives the chance to quantitatively determine the BAC. The results have confirmed that the method is linear at concentrations ranging from 1.0×10-6 mol/L to 5.0 ×10 -6 mol/L. Depending calibration equation ΔA/Δt, min-1 has the form: tgα = 5093 c + 0.0075 (r = 0,999). LOD was 0.4× 10-6 mol/L. The method was successfully applied to determine BAC in nasal pharmaceutical preparation «Aqua-rinosol». The recovery was 99.9%, RSD = 2.7 % (n=5, P=0.95). This method is beneficial because the high sensitivity and selectivity can be achieved using the relatively simple technical equipment.

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DOI: http://dx.doi.org/10.18007/gisap:msp.v0i13.1658

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